O-32: Nitric Oxide and Asymmetric Dimethylarginine Levels of Serum and Peritoneal Fluid in Women with Endometriosis

Background: Endometriosis is a common gynecological syndrom. Its etiology is still not fully understand .A great number of studies reported increase NO concentration, with altered peritoneal immune defence reaction, was involved in pathogenesis of endometriosis. Asymmetric Dimethylarginine is an endogenous competitive inhibitor of NO synthase. Elevated ADMA level is associated with reduced systemic NO production. This study was design to compare NO and ADMA concentration in serum and peritoneal fluid of patients with and without endometriosis. Previosly, we reported this result in 9th congress of the european society of Gyn in Denmark with 89 subject. This result according sample size, 189 patients is ready. Materials and Methods: This study was approved by Ethical Committee of Isfahan University of Medical Sciences. Women who were subject to laparoscopy for the evaluation of infertility or pelvic pain at the Isfahan Fertility and Infertility Center were considered. The patients with hypertension, coronary arterial diseases, diabetes, renal diseases, active pelvic inflammatory disease or polycystic ovarian syndrome were excluded. After laparoscopy. In a case - control study 88 women were allocated to two groups, according to their laparoscopic results. The first group, women with (n=90) and the second group, without (n=89) endometriosis. The venous blood samples were obtained from all patients before induction of anesthesia. The samples were centrifuged and the serums were stored at -20°C until measurement. The peritoneal fluid samples were collected from pelvis before any manipulation. The bloody fluids were excluded. The peritoneal fluid samples also were centrifuged and the supernatant were stored at -20°C until measurement. The serum and peritoneal levels of nitrite (stable NO metabolite) were measured using a colorimetric assay kit that involves the Griess reaction (promega corporation USA). The serum and peritoneal fluid levels of ADMA were measured using enzyme immunoassay kit (DLD Dignostica Gmbh Germany). Then, absorbance was measured by a microreader in 540 nm wavelength. The samples nitrite concentration was determined by comparison to nitrite standard reference curve. Data are expressed as mean ± SEM. Unpaired t tests was applied to compare the parameters between the groups. Value of p<0.05 was considered. Results: There is significant difference in pf level of No between two groups. There is no significant difference in pf level of ADMA between two groups. Conclusion: The elevated level of No in PF but not in serum implaying a possible role of NO in the pathogenesis of endometriosis. This results support that pelvic inflammation is a consequence of endometriosis.